Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

K27ac marks_male mouse liver 27 h TCPOBOP_liver1_G150_M4

strain

CD-1 (Charles River)

treatment

3 mg/kg TCPOBOP_27 h

tissue

liver

Sex

male

age

7 weeks

antibody

K27ac (ab4729)

crosslinking method

Crosslinked frozen whole tissue

mouse id number

XE50_D1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using antibodies against K4me1 (ab8895), K4me3 (ab8580), and K27ac (ab4729). NEBNext Ultra DNA library prep kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

18344229

Reads aligned (%)

97.1

Duplicates removed (%)

4.2

Number of peaks

17699 (qval < 1E-05)

mm9

Number of total reads

18344229

Reads aligned (%)

97.0

Duplicates removed (%)

4.7

Number of peaks

17702 (qval < 1E-05)