Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

K27ac marks_male mouse liver 3 h vehicle_liver2_G121_M8

strain

CD-1 (Charles River)

treatment

Vehicle (1% DMSO in corn oil)_ 3 h

tissue

liver

Sex

male

age

7 weeks

antibody

K27ac (ab4729)

crosslinking method

Crosslinked nuclei

mouse id number

XE37_A37

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using antibodies against K4me1 (ab8895), K4me3 (ab8580), and K27ac (ab4729). NEBNext Ultra DNA library prep kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

26750286

Reads aligned (%)

95.7

Duplicates removed (%)

3.9

Number of peaks

24531 (qval < 1E-05)

mm9

Number of total reads

26750286

Reads aligned (%)

95.6

Duplicates removed (%)

4.2

Number of peaks

24517 (qval < 1E-05)