Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Larvae

Cell type

Eye discs

NA

NA

Sample

source_name

Eye imaginal discs, chromatin IP against H3K27ac

genotype

wt

genetic background

Canton S

tissue

eye imaginal discs

developmental stage

third instar larvae

chip antibody

anti-H3K27ac (Abcam , catalog number ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin from Drosophila third instar larvae wing/eye discs was immunoprecipitated with 2 ug of antibody using standard protocols. Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

dm6

Number of total reads

35900825

Reads aligned (%)

56.4

Duplicates removed (%)

50.9

Number of peaks

13054 (qval < 1E-05)

dm3

Number of total reads

35900825

Reads aligned (%)

56.5

Duplicates removed (%)

50.0

Number of peaks

12988 (qval < 1E-05)