Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

ChIP_H3K27ac_CDK19KD

cell line

MDA-MB231

cell type

breast cancer cells

genotype/variation

CDK19 knockdown

chip antibody

H3K27ac (Active Motif #39133)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP, unfixed cells were clarified after enzymmatically digesting and lysing cells, then histone-DNA complexes were isolated with antibody. Libraries were prepared with Illumina's NEXTERA XT DNA library prep kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

21175224

Reads aligned (%)

94.0

Duplicates removed (%)

8.0

Number of peaks

7919 (qval < 1E-05)

hg19

Number of total reads

21175224

Reads aligned (%)

93.8

Duplicates removed (%)

8.3

Number of peaks

7901 (qval < 1E-05)