Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

ChIP_H3K27ac_Control

cell line

MDA-MB231

cell type

breast cancer cells

genotype/variation

EV-control

chip antibody

H3K27ac (Active Motif #39133)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP, unfixed cells were clarified after enzymmatically digesting and lysing cells, then histone-DNA complexes were isolated with antibody. Libraries were prepared with Illumina's NEXTERA XT DNA library prep kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

65427131

Reads aligned (%)

92.7

Duplicates removed (%)

18.7

Number of peaks

30637 (qval < 1E-05)

hg19

Number of total reads

65427131

Reads aligned (%)

92.4

Duplicates removed (%)

19.3

Number of peaks

30537 (qval < 1E-05)