GSM2781950: H3 ChIP-seq in senescent cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3
Cell type
Cell type Class
Lung
Cell type
WI-38
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Chromatin immunoprecipitation using H3 antibodies in senescent WI38 hTERT RAF1-ER cells
cell line
WI38 hTERT RAF1-ER
state
senescent
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated and chromatin was sonicated and precleared. The H2A.Z or H3 histone-DNA complexes were isolated using antibody against H2A.Z (Abcam, ab4174) or using antibody against H3 (Abcam, ab1791) . The immunoprecipitated DNA was then purified and 10 ng was sent to BGI (HONG KONG) or EMBL GeneCore (Heidelberg, Germany) to perform library construction and sequencing. Library construction and sequencing was performed by BGI TECH SOLUTIONS (HONGKONG) for the first replicates of H2A.Z ChIP-Seq in proliferation and in senescence, and at EMBL GeneCore for the second replicates of H2A.Z ChIP-Seq in proliferation and in senescence as well as for the H3 ChIP-Seq in proliferation and in senescence.