Lysates were clarified from sonicated fixed cells and histone-DNA complexes were isolated with antibody. Samples were prepared using the ChIP-Seq DNA Sample Prep Kit (illumina, cat#: IP-102-1001) according to manufactures instructions. In the final enrichment step of the protocol 12 cycles were performed to amplify the library. Samples were sequenced on the illumina HiSeq 2000 sequencer on a single read flowcell performing 50 sequencing cycles. Each sample was sequenced on 1 lane of the flowcell.