Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2A.Z

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse, in vitro activated B cells
antibody
H2AZ
cell type
in vitro activated B cells
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% paraformaldehyde at 37C for 10 minutes followed by quenching with glycine and sonnication. Chromatin fragments were then immunoprecipitated with an anti-H2AZ antibody following standard protocols. Immunoprecipitates were processed according to Illumina's standard protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
68235868
Reads aligned (%)
97.6
Duplicates removed (%)
34.8
Number of peaks
90322 (qval < 1E-05)

mm9

Number of total reads
68235868
Reads aligned (%)
97.5
Duplicates removed (%)
34.8
Number of peaks
90332 (qval < 1E-05)

Base call quality data from DBCLS SRA