Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Plasmacytoma

MeSH Description

Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.

Sample

source_name

mouse plasmacytoma PCT4961

antibody

H3K27Ac

cell type

plasmacytoma PCT4961

strain

BALB/c

genotype

AID-/- BclXL

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% paraformaldehyde at 37C for 10 minutes followed by quenching with glycine and sonnication. Chromatin fragments were then immunoprecipitated with an anti-H3K27Ac antibody following standard protocols. Immunoprecipitates were processed according to Illumina's standard protocol

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

6533535

Reads aligned (%)

91.6

Duplicates removed (%)

15.6

Number of peaks

10744 (qval < 1E-05)

mm9

Number of total reads

6533535

Reads aligned (%)

91.5

Duplicates removed (%)

15.6

Number of peaks

10756 (qval < 1E-05)