Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me2

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse, in vitro activated B cells
antibody
H3K36me2
cell type
in vitro activated B cells
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% paraformaldehyde at 37C for 10 minutes followed by quenching with glycine and sonnication. Chromatin fragments were then immunoprecipitated with an anti-H3K36me2 antibody following standard protocols. Immunoprecipitates were processed according to Illumina's standard protocol

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
5334233
Reads aligned (%)
76.6
Duplicates removed (%)
2.3
Number of peaks
25 (qval < 1E-05)

mm9

Number of total reads
5334233
Reads aligned (%)
76.5
Duplicates removed (%)
2.5
Number of peaks
22 (qval < 1E-05)

Base call quality data from DBCLS SRA