GSM2779135: H2BGFP week4 Het rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Cardiovascular
Cell type
Cardiomyocytes
NA
NA
Attributes by original data submitter
Sample
source_name
heart
strain
C57BL/6
tissue
heart
cell type
isolated adult ventricular cardiomyocytes
developmental stage
adult
genotype
WT
chip antibody
GFP (Rockland, 600-101-215)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cardiomyocytes were isolated from the adult mice heart according to previous protocol, and the cardiomyocytes were fixed and then lysed. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the ChIP library preparation Kit (NEBE7370). Briefly, DNA was end-repaired. The blunt, phosphorylated ends were treated with TA ligase Master mix and NEBNext Adaptor for Illumina. After adaptor ligation, DNA was PCR amplified with Illumina primers for 15 cycles, the DNA library was purified and the size of DNA fragments were selected by AMPure XP beads. The libraries were then sent to be sequenced by Hiseq 2500 PE