Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

antibody

Anti-H3K4me3 Millipore (#07-473)

strain

C57BL/6J

genotype

Spo11 Gal/Gal

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates from mouse testis cells were crosslinked, sonicated and immunoprecipitated with anti-DMC1 or anti-H3K4me3 antibodies. Size selection of library fragments of 150 - 250 bp was performed after PCR amplification. SSDS samples were denatured and quickly renatured before adapter ligation.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

28859308

Reads aligned (%)

80.2

Duplicates removed (%)

13.3

Number of peaks

41256 (qval < 1E-05)

mm9

Number of total reads

28859308

Reads aligned (%)

80.1

Duplicates removed (%)

13.4

Number of peaks

41234 (qval < 1E-05)