Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

strain

C57BL/6J

tissue

liver

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was collected and flash frozen before sonication with the Covaris M2. Lysates were clarified and protein-DNA complexes were immunoprecipitated with H3K4me3 antibody. Libraries were prepared according to standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

72657453

Reads aligned (%)

87.1

Duplicates removed (%)

9.3

Number of peaks

533 (qval < 1E-05)

mm9

Number of total reads

72657453

Reads aligned (%)

87.0

Duplicates removed (%)

9.3

Number of peaks

568 (qval < 1E-05)