Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNAREnz_BRD4
cell line
LNAR'
chip antibody
Sigma HPA015055
treatment
Enz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP protocol outlined in methods of paper; Tumors harvested and flash frozen in liquid nitrogen, RNA was extracted via lysing in MP Biomedical Lysing Matrix tubes in homogenizer, then Trizol extraction, followed by cleanup using Qiagen RNEasy columns ChIP-seq and RNA-seq libraries prepared using standard KAPA Hyper Library and Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
20371764
Reads aligned (%)
85.2
Duplicates removed (%)
2.7
Number of peaks
933 (qval < 1E-05)

hg38

Number of total reads
20371764
Reads aligned (%)
87.0
Duplicates removed (%)
1.6
Number of peaks
843 (qval < 1E-05)

Base call quality data from DBCLS SRA