Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Prostate

Cell type

LREX

NA

NA

Sample

source_name

LNAREnz_H3K27me3

cell line

LREX'

chip antibody

Millipore 07-449

treatment

Enz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP protocol outlined in methods of paper; Tumors harvested and flash frozen in liquid nitrogen, RNA was extracted via lysing in MP Biomedical Lysing Matrix tubes in homogenizer, then Trizol extraction, followed by cleanup using Qiagen RNEasy columns ChIP-seq and RNA-seq libraries prepared using standard KAPA Hyper Library and Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

23161904

Reads aligned (%)

85.8

Duplicates removed (%)

2.2

Number of peaks

728 (qval < 1E-05)

hg19

Number of total reads

23161904

Reads aligned (%)

85.3

Duplicates removed (%)

3.1

Number of peaks

841 (qval < 1E-05)