Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

Lin- bone marrow

strain

C57BL/6

cell type

HMM cells with HOXA9 inactivation

viral transduction

Hoxa9/Meis1

days in culture

25-31

culture condition

Myeloid (10ng/mL IL3) + 4OHT wash off

chip antibody

None

tissue

Lin- bone marrow

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were collected from sonicated cells. Chromatin fragments were isolated with antibody, RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

39343579

Reads aligned (%)

96.5

Duplicates removed (%)

18.4

Number of peaks

471 (qval < 1E-05)

mm9

Number of total reads

39343579

Reads aligned (%)

96.3

Duplicates removed (%)

18.5

Number of peaks

552 (qval < 1E-05)