Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Blood

cell line

THP-1

cell type

AML Cell Line

chip antibody

H3K9me3 ThermoFisher 49-1008

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from Mnase treated chromatin and histone-DNA complexes were isolated with antibody. Standard illumina library construction

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

19810835

Reads aligned (%)

98.2

Duplicates removed (%)

7.4

Number of peaks

420 (qval < 1E-05)

hg19

Number of total reads

19810835

Reads aligned (%)

96.8

Duplicates removed (%)

10.2

Number of peaks

388 (qval < 1E-05)