ChIP-Atlas: SRX3152286

Antigen

Cell type Class: Neural
Cell type: Hippocampus
MeSH Description: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: ChIP-seq libraries were prepared using NEXTflex ChIPSeq Kit (#5143-02, Bioo Scientific) following the manufacturer’s protocol (V12.10) with some modifications. Briefly, 10 ng of ChIP-enriched DNA or Input DNA were end repaired using T4 DNA polymerase, Klenow DNA polymerase and T4 PNK, then size selected and cleaned-up using Agencourt AMPure XP beads (#A63881, Beckman). A single ‘A’ nucleotide was added to the 3′ ends of the blunt DNA fragments with a Klenow fragment (3′ to 5′exo minus). The ends of the DNA fragments were ligated to double-stranded barcoded DNA adapters (NEXTflex ChIP-Seq Barcodes-6, #514120, Bioo Scientific) using T4 DNA Ligase. The ligated products were enriched by PCR [2 min at 98°C; (30 s at 98°C, 30 s at 65°C, 60 s at 72°C) x 14 cycles; 4 min at 72°C] and cleanedup using Agencourt AMPure XP beads.