Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC HUES6

NA

NA

Sample

source_name

HUES6 human embryonic stem cell line

tissue

embryonic stem cells

gender

female

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA was purified from tissue, sorted cells or nuclei using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. MethylC-Seq libraries were constructed as descibed previously [Lister R. et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells, Nature 471, 68-73 (2011)], except library amplification was performed with either PfuTurboCx Hotstart DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) or KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems, Woburn, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

431386625

Reads aligned (%)

90.7

Coverage rate (×)

4.1

Number of hyper MRs

172321 (qval < 1E-05)

hg19

Number of total reads

431386625

Reads aligned (%)

90.9

Coverage rate (×)

4.6

Number of hyper MRs

162625 (qval < 1E-05)