Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Others

Cell type

Thyroid Gland

MeSH Description

A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.

Sample

source_name

WT GFP-ChIP

strain background

C57BL/6

genotype/variation

wild type

diet

low-iodine diet (LID) for 2 weeks

tissue

thyroid gland

chip antibody

agarose crosslinked with a GFP-binding peptide

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq, Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with agarose crosslinked with a GFP-binding peptide. For RNA-Seq, total RNAs from thyroid gland were extracted using a RNAqueous-Micro kit (ThermoFisher Scientific) ChIPed DNA was amplified using a TruSeq RNA Library prep kit. For RNA-Seq, extracted RNA was amplified using a TruSeq RNA Library preparation kit

Sequencing Platform

instrument_model

Illumina MiSeq

mm10

Number of total reads

7177464

Reads aligned (%)

99.1

Duplicates removed (%)

6.2

Number of peaks

104 (qval < 1E-05)

mm9

Number of total reads

7177464

Reads aligned (%)

99.0

Duplicates removed (%)

6.3

Number of peaks

85 (qval < 1E-05)