GSM2756685: ES Spt6i input chip; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ES-E14TG2a
developmental stage
embryonic stem cell, blastocyst derived
strain
E14
transfection
Spt6 siRNA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPSeq,cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments. Lysates were cleared from sonicated nuclei and used for immunoprecipitation. For RNASeq, RNA was extracted according to TRIzol protocol (invitrogen). For ChIPSeq, 10ng of immuno-precipitated DNA fragments were used to prepare ChIP-seq libraries with Ovation SP Ultralow DR Multiplex system (Nugen) following the manufacturer’s protocol. the NEBNext RNA library prep kit (New England BioLabs) and Ovation SP Ultralow DR Multiplex system (Nugen) were used to process RNA-Seq samples following the manufacturer’s protocol.