Cells were kept in DMEM supplemented with 2 μg/ml Doxycycline, for induction the cells were grown for 48h in DMEM without Doxycycline
antibody
CTCFL S6
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated and sheared using BioRuptor (Diagenode). ChIPs were performed using a One-Day Protocol as described by Nelson et al. (2006 / Nature Protocols / DOI: 10.1038/nprot.2006.27 Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions