Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa, nonCSC, Pol2

cell type

HeLa cells transfected with ZsGreen-cODCdegron

chip antibody

Pol2 (abcam, ab5131)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Part# 15023092 Rev. B).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

37468714

Reads aligned (%)

97.8

Duplicates removed (%)

6.9

Number of peaks

1610 (qval < 1E-05)

hg19

Number of total reads

37468714

Reads aligned (%)

96.8

Duplicates removed (%)

8.7

Number of peaks

997 (qval < 1E-05)