Antigen

Antigen Class

Histone

Antigen

H3K9ac

Cell type

Cell type Class

Kidney

Cell type

Renal fibroblasts

NA

NA

Sample

source_name

renal fibroblast

cell type

renal fibroblast

passage

4-10

strain

C57BL/6

genotype

Wild type

chip antibody

H3K9ac (Takara, MA305B)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. All of the protocols for Illumina/Solexa sequence preparation, sequencing and quality control were provided by Illumina.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

11711842

Reads aligned (%)

73.1

Duplicates removed (%)

10.3

Number of peaks

12697 (qval < 1E-05)

mm9

Number of total reads

11711842

Reads aligned (%)

73.0

Duplicates removed (%)

10.4

Number of peaks

12710 (qval < 1E-05)