Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
ERa AZD2014 treatment
cell type
Breast cancer
passages
p22
cell line
MCF7
chip antibody
ERa sc-543
agent
Treatment with AZD201

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked for 10min using 1% formaldehyde. After sonication, chromatin immunoprecipitation was performed using a polyclonal ERa antibody over night. After decrosslinking at 65 C over night followed by RNase A and proteinase K treatment, immunoprecipitated DNA was purified by phenol-chloroform extraction. DNA was prepared for Illumina sequencing on the HiSeq 2500 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
35214403
Reads aligned (%)
91.1
Duplicates removed (%)
6.4
Number of peaks
19689 (qval < 1E-05)

hg38

Number of total reads
35214403
Reads aligned (%)
93.1
Duplicates removed (%)
5.0
Number of peaks
19650 (qval < 1E-05)

Base call quality data from DBCLS SRA