Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Blood

Cell type

Tfh

NA

NA

Sample

source_name

non-Tfh cells

strain

T-bet-Cre-Rosa26-loxP-STOP-loxP-tdTomato mice on C57BL/6 background

tissue

draining LN

condition

CXCR5lowPD1lowToma+

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

For RNA-Seq: the total RNAs were purified using Qiagen's miRNeasy micro kit (Qiagen cat# 217084) plus Qiagen’s DNase set (Qiagen cat# 79254) for on-column DNase digestion option. PolyA-tailed RNAs were purified from pure total RNA using Dynabeads® mRNA DIRECT™ Kit (ambion life technologies cat# 61012). DNase-Seq followed the protocols described previously in (PMID: 26605532). Library constructions RNA-Seq and Dnase-Seq follow the protocols described previously in (PMID: 19528076) and (PMID: 26605532)

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

47336063

Reads aligned (%)

87.2

Duplicates removed (%)

27.7

Number of peaks

16958 (qval < 1E-05)

mm9

Number of total reads

47336063

Reads aligned (%)

87.0

Duplicates removed (%)

27.7

Number of peaks

16938 (qval < 1E-05)