Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Others

Cell type

Tendons

MeSH Description

Fibrous bands or cords of CONNECTIVE TISSUE at the ends of SKELETAL MUSCLE FIBERS that serve to attach the MUSCLES to bones and other structures.

Sample

source_name

tail tendon

tissue

tail tendon

strain

C57BL/6

genotype

WT

age

3-week-old

chip antibody

H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27ac antibody. Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit(E7645) following manufacture instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

16219256

Reads aligned (%)

98.9

Duplicates removed (%)

2.2

Number of peaks

21464 (qval < 1E-05)

mm9

Number of total reads

16219256

Reads aligned (%)

98.8

Duplicates removed (%)

2.2

Number of peaks

21461 (qval < 1E-05)