Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

cervical carcinoma

cell type

HeLa Kyoto

RNAi

Wapl/Pds5A/B RNAi

genotype

Wapl/Pds5A/B depletion

chip antibody

CTCF

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq: Cells were cross-linked and scrapped of the plate. The DNA was fragmented and the protein of interest was immunoprecipitated with indicated antibodies; Adaptors were ligated to the 5' and 3' ends and the library was prepared for Illumina sequencing by a qPCR amplification step. ChIP as well as Hi-C libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

30908338

Reads aligned (%)

98.5

Duplicates removed (%)

6.2

Number of peaks

42938 (qval < 1E-05)

hg19

Number of total reads

30908338

Reads aligned (%)

97.8

Duplicates removed (%)

7.6

Number of peaks

43490 (qval < 1E-05)