GSM2747763: ChIP-Seq: SMC3 WAPL+PDS5-depleted rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMC3
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
cervical carcinoma
cell type
HeLa Kyoto
RNAi
Wapl/Pds5A/B RNAi
genotype
Wapl/Pds5A/B depletion
chip antibody
SMC3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Cells were cross-linked and scrapped of the plate. The DNA was fragmented and the protein of interest was immunoprecipitated with indicated antibodies; Adaptors were ligated to the 5' and 3' ends and the library was prepared for Illumina sequencing by a qPCR amplification step. ChIP as well as Hi-C libraries were prepared for sequencing using standard Illumina protocols.