The chromatin was digested with MNase. After IP, stringent washes, RNase and proteinase K treatment, DNA was extracted using PCR purification kit (QIAGEN). ChIP-seq library was prepared with KAPA hyperprep kit (KAPA biosystems) for the single read Illumina HiSeq 1500 platform. ChIP-seq library were prepared with KAPA hyperprep kit (KAPA biosystems) for the single read Illumina HiSeq 1500 platform.