We used 10 wing discs of each genotype (regeneration and control) and condition (0h, 15h, 25h) as well as third instar larva (L3). Samples were lysed in Lysis Buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40) by gently pipetting. Lysates were centrifuged for 10min at 500g to isolate the nuclei. Nuclei were resuspended and incubated for 30 min at 37⁰C in transposition reaction mix (Illumina). Right after the transposition reaction, samples were purified using Qiagen MinElute Kit and eluted in Elution Buffer (10mM Tris buffer, pH8). For library preparation we amplified the transposed DNA fragments by running a conventional PCR (5 min at 72⁰C, 2.5 min at 95 ⁰C, thermoycling 13 cycles 20 sec at 98⁰C, 15 sec at 63⁰C and 1 min at 72⁰C) with Nextera barcoded primers. Libraries were purified using Qiagen PCR CleanUP Kit and eluted in Elution Buffer. Illumina Nextera