Cells were fixed with 1% formaldehyde and isolated nuclei were sonicated to the desired fragment size. For H2Aubq in wild-type and Aurkb KO cells, unfixed chromatin was prepared from isolated nuclei digested with Micrococcal Nuclease to di-/mono-nucleosome. Pre-coupled antibodies-beads were used to immunoprecipitated chromatin bound proteins/histone marks. After DNA purification, a total of 10 ng immunoprecipitated input was used for the library preparation with NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB) and Multiplexing Sample Preparation Oligonucleotide Kit (Illumina)