GSM2742105: H3K9me3 visceral Repl1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Adipocyte
Cell type
Visceral adipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
visceral adipocytes
chip antibody
H3K9me3, abcam ab8898
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP mature adipocyte cells were crosslinked with 1% formaldehyd for 10 min and sonicated to 200 bps. Genomic DNA was extracted using antibodies for H3K4me3, H3K9me3 and H3K27ac. The libraries for ChIP-Seq were preparated using the MicroPlex Library preparation kit (Diagenode) and purified with Agencourt AMPure XP beads (Beckman Coulter Life Sciences, Krefeld, Germany).