Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549 lung cancer cell line

cell type

A549 lung cancer cell line

chip antibody

H3K27me3 (Cell Signaling Technology,#9733)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation and DNA extraction were performed using SimpleChIP Enzymatic Chromatin IP Kit (9003, Cell Signaling) following manufacturer’s instructions. ChIP-seq libraries were prepared with TruSeq DNA sample prep kit using standard protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

93773669

Reads aligned (%)

97.1

Duplicates removed (%)

11.0

Number of peaks

565 (qval < 1E-05)

hg19

Number of total reads

93773669

Reads aligned (%)

96.7

Duplicates removed (%)

11.2

Number of peaks

466 (qval < 1E-05)