Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2_H3K27ac CHIP-seq in shNS

cell line

HepG2

cell type

liver cancer cell line

tranduced with

shNS

chip antibody

Histone H3K27ac antibody (pAb)

chip antibody vendor

Active Motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 0.3% formaldehyde for 30 minutes at 4 degrees prior to processing for ChIP using protocol from Raab et al 2015. Kapa DNA hyperpep kit

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

16453584

Reads aligned (%)

91.0

Duplicates removed (%)

1.5

Number of peaks

19336 (qval < 1E-05)

hg19

Number of total reads

16453584

Reads aligned (%)

90.2

Duplicates removed (%)

1.6

Number of peaks

19368 (qval < 1E-05)