Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Neural

Cell type

Hippocampus

MeSH Description

A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Sample

source_name

hipocampal tissue from adult mice

genotype

mutant (KO Kdm5c)

Sex

male

age

Adult mice (2-4-month-old)

strain

pure C57BL/6J background

antibody

H3K4me1 (Abcam, #ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody against H3K4me3 (Millipore, #07-473), H3K4me1 (Abcam, #ab8895), or Kdm5C (Iwase et al., 2016). Single-end DNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq V4 Sample Prep Kit). Service provided by CRG (Barcelona. Spain)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

48924123

Reads aligned (%)

98.5

Duplicates removed (%)

15.6

Number of peaks

479 (qval < 1E-05)

mm9

Number of total reads

48924123

Reads aligned (%)

98.2

Duplicates removed (%)

15.6

Number of peaks

551 (qval < 1E-05)