Antigen

Antigen Class

Histone

Antigen

H3K122ac

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

siYT_H3K122ac, MDA-MB-231

cell line

MDA-MB-231 breast cancer cells

chip antibody

Histone H3 Acetyl K122 (abcam, ab33309, lot GR284790-3)

treatment

siYAPTAZ

genotype/variation

YAP/TAZ depletion

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was sheared by sonication and immunoprecipitated with antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

62777367

Reads aligned (%)

98.7

Duplicates removed (%)

11.4

Number of peaks

58258 (qval < 1E-05)

hg19

Number of total reads

62777367

Reads aligned (%)

98.5

Duplicates removed (%)

11.7

Number of peaks

58230 (qval < 1E-05)