GSM2736147: DMSO Pol2 rep1 (chip-seq); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
DMSO_Pol2, MDA-MB-231
cell line
MDA-MB-231 breast cancer cells
chip antibody
RNA polymerase II 8WG16 (abcam, ab817, lot GR271062-1)
treatment
DMSO
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sheared by sonication and immunoprecipitated with antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit