Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Others

Cell type

Tail fibroblast

NA

NA

Sample

source_name

neonatal tail tip fibroblasts, Hotair knockout

strain

backcross to C57BL6 with over 97% similarity

cell type

neonatal tail tip fibroblasts

genotype/variation

Hotair knockout

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Extracted DNA was RNase treated and purified using Qiagen column, and sequencing libraries were made following Illumina’s protocol. For ChIP-seq libraries, chromatin was solubilized and isolated with corresponding antibodies, and the libraries were made following Illumina protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

39464605

Reads aligned (%)

96.8

Duplicates removed (%)

7.3

Number of peaks

1714 (qval < 1E-05)

mm9

Number of total reads

39464605

Reads aligned (%)

96.7

Duplicates removed (%)

7.4

Number of peaks

1730 (qval < 1E-05)