DPdouble positive thymocytes derived from in vitro expanded bone marrow cells transduced with Nup98-HoxB4, grown with IL7 and DLL4 (Notch signaling) and injected into Rag2-/- mouse
cell type
double positive thymocytes derived from in vitro expanded bone marrow cells transduced with Nup98-HoxB4, grown with IL7 and DLL4 (Notch signaling) and injected into Rag2-/- mouse
strain
B6
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Libraries were prepared according to Illumina's instructions accompanying the Agilent_DNA _Kit _100. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were generated and sequenced on the Genome Analyzer following the Illumina manufacturer's protocols.