Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

HSC in vitro expanded bone marrow cells transduced with Nup98-HoxB4

cell type

in vitro expanded bone marrow cells transduced with Nup98-HoxB4

strain

B6

chip antibody

anti H3K27me3 antibody (Cell Signaling #9733)

chip antibody manufacturer

Cell Signaling

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Libraries were prepared according to Illumina's instructions accompanying the Agilent_DNA _Kit _100. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were generated and sequenced on the Genome Analyzer following the Illumina manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

45026568

Reads aligned (%)

85.4

Duplicates removed (%)

72.1

Number of peaks

249 (qval < 1E-05)

mm9

Number of total reads

45026568

Reads aligned (%)

85.3

Duplicates removed (%)

72.3

Number of peaks

280 (qval < 1E-05)