Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
cell type
mouse embryonic stem cells
cell line
Blm-/-;Ago1,2,3,4-/-;CreERT2
antibody
Mouse monoclonal RNAPII-CTD abcam ab817

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and bound DNA fragments were isolated with antibody. Genomic DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
8896885
Reads aligned (%)
97.2
Duplicates removed (%)
17.9
Number of peaks
4108 (qval < 1E-05)

mm9

Number of total reads
8896885
Reads aligned (%)
96.9
Duplicates removed (%)
18.1
Number of peaks
4108 (qval < 1E-05)

Base call quality data from DBCLS SRA