SRX3056457
GSM2729833: E7 H3K27me3 ChIP; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
cell type
mouse embryonic stem cells
cell line
Blm-/-;Ago1,2,3,4-/-;CreERT2;hAgo2fl
antibody
Mouse monoclonal H3K27me3 abcam ab6002

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and bound DNA fragments were isolated with antibody. Genomic DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
19673499
Reads aligned (%)
97.2
Duplicates removed (%)
19.4
Number of peaks
439 (qval < 1E-05)

mm9

Number of total reads
19673499
Reads aligned (%)
97.0
Duplicates removed (%)
19.5
Number of peaks
431 (qval < 1E-05)

Base call quality data from DBCLS SRA