ChIP was performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).