ChIP-Atlas: SRX3050831

Antigen

Cell type Class: Neural
Cell type: Retina
MeSH Description: The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: ATAC-seq library preparation was performed as described (Buenrostro et al., 2013) with minor modifications. Briefly, for each experiment, 2 × 105 - 5 × 105 dissociated retinal cells were washed twice with PBS (Lonza, 17-516F) containing Proteinase Inhibitor Cocktail (Sigma-Aldrich P8340), incubated with 50 μL of lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2 and 0.3% IGEPAL CA-630) on ice for 10 min and centrifuged at 500 g for 10 min at 4°C. After centrifugation, the pellet was immediately incubated with 50 μL of Tn5 transposition reaction mix (25 μL TD buffer, 2.5 μL TDE1 (Nextera DNA Library Prep Kit, Illumina, FC-121-1030) and 22.5 μL nuclease-free water) at 37°C for 30 min. After purification with QIAGEN MinElute PCR Purification Kit (QIAGEN, 28006), transposed DNA fragments were amplified with NEBNext High-Fidelity 2 × PCR Master Mix (New England Biolabs, M0541) for 5 cycles using the following PCR conditions: 72°C for 5 min; 98°C for 30 s; and thermocycling at 98°C for 10 s, 63°C for 30 s and 72°C for 1 min. 5 μL of each library was amplified in a 25 μL qPCR reaction with KAPA SYBRFAST qPCR Kit (Kapa Biosystems, KK4603) to estimate the optimum number of additional cycles to finish amplification of the remaining 45 μL library.