GSM2720196: HBE DNase-seq rep3; Homo sapiens; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Lung
Cell type
Bronchial epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
HBE DNase-seq
donor status
healthy donor
cell type
primary human bronchial epithelial cells
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012 genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE) DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.