Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Lung

Cell type

Bronchial epithelial cells

NA

NA

Sample

source_name

HBE DNase-seq

donor status

healthy donor

cell type

primary human bronchial epithelial cells

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012 genomic DNA is digested with different concentrations of DNaseI (NEB) and purified for library construction. Total RNA is extracted by Trizol (HBE) or RNeasy (HTE) DNase-seq libraries were prepared as described in Bischof et al. Thorax. 67:385-391. 2012. RNA seq libraries prepared by standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

24492884

Reads aligned (%)

99.8

Duplicates removed (%)

10.3

Number of peaks

6973 (qval < 1E-05)

hg19

Number of total reads

24492884

Reads aligned (%)

99.8

Duplicates removed (%)

10.6

Number of peaks

6978 (qval < 1E-05)