Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP Bag1L WT cells
treatment
ETOH (4 h)
phenotype
Bag-1L WT
chip antibody
AR N-20 antibody (Santa Cruz Biotechnology; sc-816; lot # I0215)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked cells were lysed using a Sarkosyl-containing lysis buffer. Chromatin was isolated, sheared and subjected to immunoprecipitation with an anti-AR antibody. Libraries were prepared using 5 ng ChIP DNA and the ThruPLEX DNA-seq kit (Rubicon Genomics) according to the manufacturer's instructions. ChIP-seq. Libraries were sequenced on an Illumina NS500 Single-End 75 bp platform.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36281257
Reads aligned (%)
89.1
Duplicates removed (%)
10.8
Number of peaks
3463 (qval < 1E-05)

hg38

Number of total reads
36281257
Reads aligned (%)
89.7
Duplicates removed (%)
10.2
Number of peaks
3509 (qval < 1E-05)

Base call quality data from DBCLS SRA