Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFP

Cell type

Cell type Class
Pluripotent stem cell
Cell type
mESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
R1 ESC-derived NPCs
strain background
129/Ola
cell type
Neural progenitor cells
genotype/variation
R1 ESC-derived NPCs
culture conditions
NPC medium
chip antibody
GFP (Abcam, ab290)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer’s instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
35080350
Reads aligned (%)
95.5
Duplicates removed (%)
13.9
Number of peaks
2358 (qval < 1E-05)

mm9

Number of total reads
35080350
Reads aligned (%)
95.3
Duplicates removed (%)
14.6
Number of peaks
2325 (qval < 1E-05)

Base call quality data from DBCLS SRA