Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

WT IB10 ESCs

strain background

129/Ola

cell type

Embryonic stem cells

genotype/variation

WT IB10 ESCs

culture conditions

Serum/LIF

chip antibody

GFP (Abcam, ab290)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer’s instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

47267888

Reads aligned (%)

96.8

Duplicates removed (%)

16.6

Number of peaks

1044 (qval < 1E-05)

mm9

Number of total reads

47267888

Reads aligned (%)

96.7

Duplicates removed (%)

17.3

Number of peaks

1066 (qval < 1E-05)